Deoxyribonucleic acid synthesis in bacteriophage SP01-infected Bacillus subtilis. II. Purification and catalytic properties of a deoxyribonucleic acid polymerase induced after infection.

Effect of nalidixic acid on deoxyribonucleic acid synthesis in bacteriophage SPO1-infected Bacillus subtilis.

The effect of nalidixic acid on deoxyribonucleic acid (DNA) synthesis in Bacillus subtilis cells infected with bacteriophage SPO1 was studied. Nalidixic acid had little inhibitory effect on SPO1 DNA synthesis at concentrations that drastically inhibited B. subtilis DNA synthesis. Inhibition of DNA synthesis, appropriate to the concentration used, was imposed within 1 min after addition of nalid...

Enzymatic Synthesis of Deoxyribonucleic Acid. Xv. Purification and Properties of a Polymerase from Bacillus Subtilis.

Deoxycytidine triphosphatase, an enzyme induced by bacteriophage infection.

In previous communications we have brirfly dcscribrd the properties of a dcoxycytidine triphosphate-cleaving enzyme (deoxycytidine triphosphatase) which appears in Escherichia coli cells after infection with bacteriophage T2 (1, 2). The prcscnce of this enzyme was first noted because of its property of excluding dCTP’ as a substrate for the deoxyribonucleic acid polymerase present in cell-free ...

Relationship between transformation in Bacillus subtilis and infection by bacteriophage SP02.

When bacteriophage SP02 infects a Bacillus subtilis culture during or shortly after transformation, the frequency of transformants among the resulting lysogens is greatly reduced relative to that in the uninfected culture. The effect can occur after the deoxyribonucleic acid has been taken up and covalently attached to recipient deoxyribonucleic acid.

Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.

The synthesis of T2 bacteriophage deoxyribonucleic acid is now known to depend on the development of a series of new enzymes which are undetectable in uninfected Escherichia coli (2-7). An augmented rate of deoxyribonucleic acid synthesis also occurs (8) which is accompanied by an approximately lo-fold increase in polymerase activity in extracts of these cells, as compared with extracts of unin...